and Healthspan Core
This Core facilitates state-of-the-art experimentation in three of the most common invertebrate model organisms used to study the basic biology of aging: the budding yeast Saccharomyces cerevisiae, the nematode Caenorhabditis elegans, and the fruit fly Drosophila melanogaster. Our overarching goal is to provide training, resources, and access to experimental methods in aging research that would otherwise be unavailable or difficult to obtain for many investigators. Core personnel work closely with Core users to develop the optimal approaches and experimental design for their specific project and the scientific questions they wish to address.
The Core provides access to specialized libraries, equipment, and assays useful for studying aging in invertebrate models. This includes lifespan analysis in yeast, worms, and flies, as well as several important measures of healthspan in these organisms. Specialized Core equipment that is available for users includes a Bioscreen C MBR machine, a Zeiss Lumar Fluorescence Microscope, and a computer-controlled system for dynamically controlling oxygen levels in a temperature-controlled incubator system that we have used for hypoxia and hyperoxia sensitivity experiments in yeast, worms, and fruit flies. This system can maintain oxygen at a constant level between 0.005% and 100% O2 or can intermittently change the oxygen levels continuously for as long as necessary.
In addition to these resources, a generous $450,000 grant from the Murdock Charitable Trust has allowed the Core to purchase a COPAS Biosort Wormsorter.
Drosophila melanogaster lab cultures.
Photo by Trick17 - Own work. Licensed under Creative Commons Attribution 3.0 via Wikimedia Commons